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US 5,492,820 AGranted
Plasmids for the production of transgenic plants that are modified in habit and yield
AssigneeInstitut Fur Genbiologische Forschung Berlin GmbH
InventorsUwe Sonnewald, Lothar Willmitzer
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Abstract
Plasmids that comprise a DNA sequence coding for a protein that modifies the metabolism of soluble sugars, for example, an inorganic pyrophosphatase agent and plant cells comprising those plasmids, for the production of transgenic plants that are modified in habit and yield arc described, the plants being modified in habit by the transfer and the expression of genes that intervene in sugar metabolism or sugar distribution inside a plant.
Claims13
A plasmid comprising the following DNA-sequences:
- a) a promoter active in plant cells;
- b) at least one DNA sequence coding for an E. coli inorganic pyrophosphatase which is linked in a sense-orientation to the 3'-end of said transcriptional promoter;
- and
- c) a transcriptional termination sequence,
- wherein when said plasmid is transferred into a plant cell thereby producing a transformed cell, said transformed plant cell expresses an additional pyrophosphatase activity in the cytosol of said transformed cell, resulting in a reduction of the concentration of free pyrophosphate and an increase in the concentration of at least one of the soluble sugars of the group consisting of UDP-glucose, glucose, fructose and sucrose in said transformed plant cell compared to an untransformed plant cell.
2 more elements
Plasmid p 35-.OMEGA.-ppase (DSM 6141).
Plasmid L 700:ppa (DSM 6733
Dependencies are inferred from the claim text.