Composition and method for enhancing detection of superoxide anion
AssigneeTrustees of Dartmouth College
InventorLorraine C. Pfefferkorn
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Abstract
A composition and method for enhancing luminol chemiluminescence assays for superoxide anion comprising an orthovanadate salt, such as sodium orthovanadate. Orthovanadate enhances photon emission when luminol is cleaved by superoxide anion. The orthovanadate salt is non-cytotoxic and non-denaturing to intact cells. A method comprises reacting luminol in the presence of an orthovanadate anion with an analyte suspected of containing superoxide anion and measuring the photon output. A kit comprises a predetermined mount of luminol and orthovanadate.
Claims8
A composition for enhancing detection of a superoxide anion, comprising an orthovanadate anion and a chemiluminescent substance which is cleavable by a superoxide salon to produce a detectable signal, said composition being non-denaturing and non-cytotoxic to intact cells.
Dependencies are inferred from the claim text.