US 5,491,225 A · PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays

Abstract

This invention provides for superior nucleic acid primers for amplification of select target regions of the genome of the genus Legionella. The invention facilitates detection of pathogenic and nonpathogenic forms of this genus. The invention further provides for processes for using the primers in template dependent nucleic acid polymerase extension reactions to amplify select target regions. Kits for the use of these primers are also provided. This invention further provides for methods of controlling the intensity of visual signal for detection of duplex formation in nucleic acid hybridization assays under high stringent conditions. This method involves the blending of different capture probes onto a solid support.

Claims16

Dependent claims

Nucleic acid polymerase primers for the amplification of subsequences of nucleic acid from Legionella species, wherein the primers are selected from the group consisting of

(a) 5'-GCATTGGTGCCGATTTGG-3', Seq. ID No. 6;
(b) 5'-GCTTTGCCATCAAATCTTTCTGAA-3', Seq. ID No. 7;
(c) 5'-GGCGACTATAGCGRTTTGGAA-3', Seq. ID No. 10;
(d) 5'-GCGATGACCTACTITCRCATGA-3', Seq. ID No. 9;
and
(e) 5'-GTTTTGCCATCAAATCTTFTTGAA-3', Seq. ID No. 23
where R represents an adenine or a guanine base and the primers having a base R are a mixture of the two primers.

4 more elements

Dependencies are inferred from the claim text.

PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays

Life of this patent

PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays

Life of this patent

Abstract

Claims16