Method for depositing metal particles on a marker
AssigneeJanssen Pharmaceutica NV
InventorsMarcus J. M. Noppe, Frank J. Konings
Life of this patent
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Abstract
An improved method for depositing metal particles on a marker which catalyzes the reduction of metal ions from a physical developer comprising a solution of metal ions, a molar excess of complexant in respect to the metal ions and a reducing agent. Said method being preferably employed for the detection of one or more components of an aggregate formed between a least one specific binding agent and its corresponding bindable substance by labelling at least one component of said aggregate with a marker and contacting said aggregate with a physical developer, whereby under influence of the marker a metal particle is formed which can be detected. Further the invention also relates to solutions and test-kits adapted for carrying out the above mentioned method.
Claims15
A method for quantitatively or qualitatively determining one or more components of an aggregate formed between at least one specific binding agent and its corresponding bindable substance which comprises labelling at least one component of said aggregate with a marker and contacting said aggregate with a physical developer whereby under influence of the marker a metal particle is formed which can qualitatively or quantitatively be determined to provide a qualitative or quantitative indication of the component or components to be determined, characterized in that the physical developer used comprises:
- (a) a solution of metal ions wherein said metal ions are selected from the group consisting of silver, gold, platinum, palladium and thallium ions:
- (b) a molar excess relative to the metal ions of a complexant having one or more nitrogen donor atoms, wherein the complexant is histidine or imidazole;
- and (c) a reducing agent.
A method for qualitatively or quantitatively determining an acceptor substance which is directly immobilized on or in a solid support which comprises binding the acceptor substance with a marker and contacting said marker with a physical developer, whereby under influence of the marker a metal particle is formed which can quantitatively or qualitatively be determined to provide a qualitative or quantitative indication of the component or components to be determined, characterized in that the physical developer used comprises;
- (a) a solution of metal ions wherein said metal ions are selected from the group consisting of silver, gold, platinum, palladium, and thallium ions;
- (b) a molar excess relative to the metal ions of a complexant having one or more nitrogen donor atoms, wherein the complexant is histidine or imidazole;
- and (c) a reducing agent.
A test kit for qualitatively or quantitatively determining one or more components of an aggregate formed between at least one specific binding agent and its corresponding bindable substance by labelling at least one component of said aggregate with a marker and contacting said aggregate with a physical developer, comprising a physical developer which comprises:
- (a) a solution containing metal ions wherein said metal ions are selected from the group consisting of silver, gold, platinum, palladium and thallium ions;
- (b) a molar excess relative to the metal ions of a complexant having one or more nitrogen donor atoms, wherein the complexant is histidine or imidazole;
- and (c) a reducing agent.
Dependencies are inferred from the claim text.