Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
AssigneeHoffmann-La Roche Inc.
InventorsDavid H. Gelfand, Alice M. Wang
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Abstract
Recombinant DNA sequences encoding the DNA polymerase activity of Pyrodictium species can be used to construct recombinant vectors and transformed host cells for production of the activity. Pyrodictium enzymes for catalyzing 3'.fwdarw.5' exonuclease activity, i.e., proofreading enzymes, are also provided. The Pyrodictium enzymes are useful in DNA amplification procedures and are not irreversibly inactivated by exposure to 100.degree. C. in a polymerase chain reaction.
Claims20
A purified protein that consists of an amino acid sequence that is SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A purified DNA consisting of a sequence that encodes an amino acid sequence that is SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A purified DNA polymerase that is encoded by pexo-Pab or pexo-Poc.
A purified DNA that encodes the DNA polymerase encoded by pexo-Pab or pexo-Poc.
Dependencies are inferred from the claim text.