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US 5,491,085 AGranted
Method of recovering peroxidase from seed hulls using a freeze-thaw technique
AssigneeEnzymol International, Inc.
InventorsAlexander R. Pokora, Mark A. Johnson
Life of this patent
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Abstract
Peroxidase can be recovered from seed hulls in an improved method using a freeze-thaw technique. First, the seed hulls are comminuted, placed into water and homogenized. Next, the homogenate is frozen then thawed. The enzyme is then recovered from the aqueous solution by conventional means. Soybean or rice seed hulls can be used in this process.
Claims9
A method for recovering peroxidase as a solution in water comprising:
- preparing a homogenate of seed hulls containing peroxidase in water, freezing said homogenate, thawing said frozen homogenate, and separating the particles from said homogenate to obtain a solution of said peroxidase in said water.
A method for recovering peroxidase as a solution in water from a plant part containing peroxidase comprising:
- preparing a homogenate of said plant part in water, freezing said homogenate, thawing said frozen homogenate, and separating the particles from said homogenate to obtain a solution of peroxidase in water.
Dependencies are inferred from the claim text.