Uses of green-fluorescent protein

A method for selecting cells expressing a protein of interest which comprises:

• (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein;
• (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest;
• and
• (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest,
• wherein DNAI and DNAII are linked.

A method for selecting cells expressing a protein of interest which comprises:

• (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein;
• (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest;
• and
• (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest,
• wherein the cells are selected from a group consisting of yeast cells, fungal cells, insect cells, nematode cells, plant or animal cells.

A method for localizing a protein of interest in a cell which comprises:

• (a) introducing into a cell a DNA molecule having DNA sequence encoding the protein of interest linked to DNA sequence encoding an Aequorea victoria green-fluorescent protein such that the protein produced by the DNA molecule will have the protein of interest fused to the Aequorea victoria green-fluorescent protein;
• (b) culturing the cell in condition permitting expression of the fused protein;
• and
• (c) detecting the location of the fluorescence of the fused protein in the cell, thereby localizing a protein of interest in a cell.