Reagents and methods for the detection and quantification of testosterone in fluid samples

A test kit for the quantification of testosterone in a test sample, said test kit comprising:

• (a) a Position 1 labeled reagent of the following formula:
• ##STR24## wherein Q is a detectable moiety, W is a linking moiety, and n is between 2 and 10, inclusively;
• and
• (b) an antibody reagent comprising antibodies which are capable of binding to testosterone and said labeled reagent.

A test kit for the quantification of testosterone in a test sample, said test kit comprising:

• (a) a Position 6 labeled reagent having the following formula:
• ##STR26## wherein Q is a detectable moiety, and W is a linking moiety, and (b) an antibody produced with a Position 1 immunogen having the following structural formula:
• ##STR27## wherein:
• (i) P is an immunogenic carrier material,
• (ii) n is between 2 to 10, inclusively,
• (iii) X is a linking moiety wherein --X--P is attached to a testosterone derivative,
• (iv) the degree of substitution of P by the testosterone derivative is between 1% to 100%, inclusively, and
• (v) said antibody is capable of binding said labeled reagent and testosterone.