Back to results
US 5,491,069 AGranted
Biomarkers of cell senescence
AssigneeThe Regents of the University of California
InventorsGoberdhan P. Dirmi, Judith Campisi, Monica Peacocke
Life of this patent
Estimating the term…
Abstract
The present invention provides a biomarker system for the in vivo and in vitro assessment of cell senescence. In the method of the present invention, .beta.-galactosidase activity is utilized as a means by which cell senescence may be assessed either in in vitro cell cultures or in vivo.
Claims14
A method for differentiating between senescent and young cells, comprising the steps of:
- a) providing:
- i) senescent cells,
- ii) young cells,
- iii) substrate for .beta.-galactosidase activity;
- and
- iv) a buffer having a pH between approximately 5.5 and 6.5;
- and
- b) exposing said senescent cells and said young cells to said buffer containing said .beta.-galactosidase substrate under conditions such that at least 90% of said senescent cells display .beta.-galactosidase activity and said young cells display no significant .beta.-galactosidase activity.
5 more elements
A method for differentiating between senescent and young cells, comprising the steps of:
- a) providing:
- i) a tissue sample suspected of containing senescent cells and young cells;
- ii) a substrate for .beta.-galactosidase activity;
- and
- iii) a buffer having a pH between approximately 5.5 and 6.5;
- and
- b) exposing said, tissue sample to said buffer containing said galactosidase substrate under conditions such that greater than 90% of said senescent cells display .beta.-galactosidase activity and less than or equal to 1% of said young cells display .beta.-galactosidase activity.
4 more elements
Dependencies are inferred from the claim text.