Anti-human ceruloplasmin monoclonal antibody
AssigneeIdemitsu Kosan Co., Ltd.
InventorsShuichi Hiyamuta, Keiko Shimizu, Akihiko Kodota
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Abstract
Disclosed is a monoclonal antibody which specifically reacts with active human ceruloplasmin as well as a method for diagnosing Wilson's disease using the same. The monoclonal antibody of the present invention specifically reacts with active human ceruloplasmin, it neutralizes the peroxidase activity of the active human ceruloplasmin upon binding thereto, and it does not specifically react with inactive human ceruloplasmin.
Claims12
A method for detecting or quantifying active human ceruloplasmin which comprises:
- contacting a sample to be assayed with an antibody, wherein said antibody specifically binds to active human ceruloplasmin, wherein said antibody neutralizes the peroxidase activity of the active human ceruloplasmin upon binding thereto, and wherein said antibody further does not bind specifically to inactive human ceruloplasmin, to form an antibody-ceruloplasmin complex;
- and
- measuring the amount of said antibody-ceruloplasmin complex so formed, thereby detecting or quantifying active human ceruloplasmin in said sample.
A method for detecting or quantifying active human ceruloplasmin in a sample which comprises:
- contacting said sample with an immobilized primary antibody which specifically finds the both active and inactive human ceruloplasmin, to form an immobilized ceruloplasmin-antibody complex;
- contacting said immobilized ceruloplasmin-antibody complex with a secondary antibody, wherein said secondary antibody specifically binds to active human ceruloplasmin, wherein said antibody neutralizes the peroxidase activity of the active human ceruloplasmin upon binding thereto, and wherein said antibody further does not bind specifically to inactive human ceruloplasmin, to form a ceruloplasmin-secondary antibody complex;
- and
- measuring the amount of said ceruloplasmin-secondary antibody complex so formed, thereby detecting or quantifying active human ceruloplasmin in said sample.
A method for detecting or quantifying active human ceruloplasmin in a sample which comprises:
- contacting said sample with an immobilized primary antibody, wherein said primary antibody specifically binds to active human ceruloplasmin, wherein said antibody neutralizes the peroxidase activity of the active human ceruloplasmin upon binding thereto, and wherein said antibody further does not bind specifically to inactive human ceruloplasmin, to form an immobilized ceruloplasmin-antibody complex;
- contacting said immobilized ceruloplasmin-antibody complex with a secondary antibody which specifically finds to both active and inactive human ceruloplasmin, to form a ceruloplasmin-secondary antibody complex;
- and
- measuring the amount of said ceruloplasmin-secondary antibody complex so formed, thereby detecting or quantifying active human ceruloplasmin in said sample.
A method for diagnosing Wilson's disease by measuring the amount of active ceruloplasmin in a sample which comprises:
- obtaining a sample of body fluid from a human patient;
- performing a method of quantifying an amount of active human ceruloplasmin in said sample according to any one of the preceding claims;
- and
- comparing the amount of active human ceruloplasmin in said sample from the patient with the amount of active human ceruloplasmin in a sample obtained from a normal human, wherein a lessened amount of active human ceruloplasmin in said sample from the patient is indicative of Wilson's disease in the patient.
Dependencies are inferred from the claim text.