Monoclonal antibodies that bind to and neutralize dimeric but not monomeric human M-CSF and methods of use thereof for detection of dimeric M-CSF
AssigneeCetus Oncology Corporation
InventorsRobert F. Halenbeck, Kirston E. Koths, Joseph W. Wrin
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Abstract
Monoclonal antibodies that bind to an apparent conformational epitope associated with recombinant or native dimeric M-CSF with concomitant neutralization of biological activity, which monoclonal antibodies are substantially unreactive with biologically inactive forms of M-CSF including monomeric and chemically derivatized dimeric M-CSF, are described. Methods of using the antibodies in assays for detection of dimeric M-CSF are also described.
Claims10
A mouse monoclonal antibody that:
- a) specifically binds to an epitope on dimeric, human M-CSF which is recognized by monoclonal antibody 382-5H4 1A8 1F6 secreted by a hybridoma on deposit with the American Type Culture Collection with Accession Number HB10027;
- b) does not measurably bind to monomeric human M-CSF;
- and
- c) neutralizes the biological activity of human M-CSF as determined by the ability of the monoclonal antibody to inhibit M-CSF bioactivity in vitro.
Monoclonal antibody 382-5H4 1A8 1F6 secreted by a hybridoma on deposit with the American Type Culture Collection with Accession Number HB10027.
A hybridoma which secretes monoclonal antibody 382-5H4 1A8 1F6, said hybridoma on deposit with the American Type Culture Collection, with Accession Number HB10027, and with the Cetus Tissue Culture Collection with Deposit Number 10523.
Dependencies are inferred from the claim text.