HTS-1 gene, a human tumor suppressor gene
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsJack H. Lichy, Peter M. Howley
Life of this patent
Estimating the term…
Abstract
A gene which is associated with tumor suppression and is localized on chromosome 11 has now been identified. The identification, localization and sequence of a gene which demonstrates differential expression in a manner that correlates with tumorigenicity suggests that this gene could potentially be used for gene therapy in cancers deleted or altered in their expression of the gene. Furthermore, a gene which is localized on chromosome 11p15, with identified polymorphisms, could be used for analysis of tumor DNA for loss of heterozygosity at chromosome 11p15. This region of chromosome 11 shows frequent loss of heterozygosity (LOH) in many human malignancies. Thus, the determination of LOH at chromosome 11p15 may be useful in predicting the prognosis of that tumor.
Claims22
A purified nucleic acid having a sequence fully complementary to a nucleic acid of Sequence I.D. No. 1 said sequence binding specifically to sequence I.D. No. 1 and not to other human DNA sequences, when subject to 0.1.times. standard saline citrate buffer, 0.1% SDS at 65.degree. C.
A method of detecting the presence of the human tumor suppressor 1 gene in a physiological specimen, said method comprising;
- (i) contacting a nucleic acid probe which is at least 12 continuous nucleotides in length and is specific for binding to human tumor suppressor 1 gene with said specimen under conditions which allow said nucleic acid probe to anneal to complementary sequences in said sample;
- and
- (ii) detecting duplex formation between said nucleic acid probe and said complementary sequences.
Dependencies are inferred from the claim text.