Immunoassay reagents and method for determining cyclosporine

A cyclosporine derivative corresponding to the formula:

• ##STR18## wherein ##STR19## represents a single or a double bond R.sub.7 is hydrogen or an acyl group of 1-6 carbon atoms;
• R.sub.8 is hydrogen or CH.sub.2 OR.sub.7 ;
• X.sub.2 is a linking group of 1-30 atoms excluding hydrogen;
• and
• F1 is a detectable moiety from a luminescent molecule selected from the group consisting of fluoresceinamines and carboxyfluoresceins.

A cyclosporine derivative corresponding to the formula:

• ##STR20## wherein ##STR21## represents a single or a double bond R.sub.7 is hydrogen or an acyl group of 1-6 carbon atoms;
• R.sub.9 is hydrogen or OR.sub.7 ;
• X.sub.1 is a linking group of 1-15 atoms excluding hydrogen;
• and
• F1 is a detectable moiety from a luminescent molecule selected from the group consisting of fluoresceinamines and carboxyfluoresceins.

A cyclosporine derivative corresponding to the formula:

• ##STR22## wherein ##STR23## represents a single or a double bond R.sub.7 is hydrogen or an acyl group of 1-6 carbon atoms;
• X.sub.1 is a linking group of 1-5 carbon atoms excluding hydrogen;
• and
• F1 is a detectable moiety from a luminescent molecule selected from the group consisting of fluoresceinamines and carboxyfluoresceins.

A cyclosporine derivative corresponding to the formula:

• ##STR24## wherein ##STR25## represents a single or a double bond R.sub.7 is hydrogen or an acyl group of 1-6 carbon atoms;
• X.sub.1 is a linking group of 1-15 atoms excluding hydrogen;
• and
• F1 is a detectable moiety from a luminescent molecules selected from the group consisting of fluoresceinamines and carboxyfluoresceins.