Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
AssigneeStratagene
InventorEric J. Mathur
Life of this patent
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Abstract
A recombinant thermostable Pyrococcus furiosus DNA polymerase is described that is deficient in 3'-5' exonuclease activity that is useful in DNA cycle sequencing reactions.
Claims9
A recombinant thermostable Pyrococcus furiosus DNA polymerase I deficient in 3' to 5' exonuclease activity, wherein said polymerase has an amino acid residue sequence represented by the formula shown in SEQ ID NO 1 from residue 1 to residue 775, with the exception of an amino acid residue substitution selected from the group consisting of (1) Ala.sup.141 /Ala.sup.143, (2) Ala.sup.214 /Ala.sup.215, and (3) Ala.sup.311 /Ala.sup.315.
A plasmid containing a gene coding for a recombinant thermostable Pyrococcus furiosus DNA polymerase I deficient in 3' to 5' exonuclease activity, wherein said polymerase has an amino acid residue sequence represented by the formula shown in SEQ ID NO 1 from residue 1 to residue 775, with the exception of an amino acid residue substitution selected from the group consisting of (1) Ala.sup.141 /Ala.sup.143, (2) Ala.sup.214 /Ala.sup.215, and (3) Ala.sup.311 /Ala.sup.315.
A bacteriophage containing a gene coding for a Pyrococcus furiosus DNA polymerase I deficient in 3' to 5' exonuclease activity, wherein said polymerase has an amino acid residue sequence represented by the formula shown in SEQ ID NO 1 from residue 1 to residue 775, with the exception of an amino acid residue substitution selected from the group consisting of (1) Ala.sup.141 /Ala.sup.143, (2) Ala.sup.214 /Ala.sup.215, and (3) Ala.sup.311 /Ala.sup.315.
Dependencies are inferred from the claim text.