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US 5,489,517 AGranted
Secretion of insulin-like growth factor-I in E. coli
AssigneeMonsanto Company
InventorsEdith Wong, Michael L. Bittner
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Abstract
A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamb or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.
Claims8
A method for producing mature IGF-I which comprises:
- causing expression of a gene in E. coli bacteria, said gene comprising sequentially in the 5' to 3' direction, a DNA sequence encoding a promoter operable in E. coli, a ribosome binding site, a DNA sequence encoding an ompF signal sequence directly joined to a DNA sequence encoding IGF-I, and a termination sequence, and recovering mature IGF-I which is secreted into the periplasmic space of the bacteria.
A method for producing IGF-I which comprises:
- causing expression of a gene in E. coli, said gene comprising sequentially in the 5' to 3' direction a DNA sequence encoding a promoter operable in E. coli, a ribosome binding site, a DNA sequence encoding an ompF signal sequence consisting of Met-Met-Lys-Arg-Asn-Ile-Leu-Ala-Val-Ile-Val-Pro-Ala-Leu-Leu-Val-Ala-Gly-Th r-Ala-Asn-Ala directly joined to a DNA sequence encoding IGF-I and a transcription termination sequence, and recovering mature IGF-I which is secreted into the periplasmic space of the E. coli.
Dependencies are inferred from the claim text.