DNA coding for growth-inhibitory factor and use thereof
AssigneeTakeda Chemical Industries, Ltd.
InventorsShoji Tsuji, Tadashi Miyatake, Yoko Uchida, Yasuo Ihara
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Abstract
A novel protein of GIF having growth-inhibitory action, which is present in the brain of normal subjects but absent in the brain of patients with Alzheimer disease, was cloned from a human-cerebral cDNA library and human genomic DNA library. Further, Escherichia coli was transformed with a vector integrated by the cDNA to express GIF. The DNA coding for the cloned GIF can be used in the genetic diagnosis and gene therapy for Alzheimer disease, and the expression of the DNA in the transformant can bring about a large production of GIF.
Claims10
A recombinant DNA coding for a protein having growth-inhibitory action (GIF) wherein said protein has the following amino acid sequence:
- (SEQ ID NO:1)
- ______________________________________ Met Asp Pro Glu Thr Cys Pro Cys Pro Ser 1 5 10 Gly Gly Ser Cys Thr Cys Ala Asp Ser Cys 15 20 Lys Cys Glu Gly Cys Lys Cys Thr Ser Cys 25 30 Lys Lys Ser Cys Cys Ser Cys Cys Pro Ala 35 40 Glu Cys Glu Lys Cys Ala Lys Asp Cys Val 45 50 Cys Lys Gly Gly Glu Ala Ala Glu Ala Glu 55 60 Ala Glu Lys Cys Ser Cys Cys Gln 65 68 ______________________________________
A recombinant DNA having a promoter region of the GIF gene promoter region (SEQ ID NO:3) has the following base sequence
- __________________________________________________________________________ GAATTCTAGA ATGAAGGGGA AGAGAGGGAG GGAAGAGCTG GGAAATACGC AAAGCGCCTT TTTCTCGACT TTCGGAGATG GTACGTGCGC GCTTCGACGG AGTGGCGGCT GCTGCGGCGA GCACGTCCCT GCGGGACCCA CGCGGGCAGT GGGCTGGCAG TCGCGGGATA GCGGCGGGGA GTGGGTCGTG CACGCGGATG CGGGGTCCCA GTGGGGGCGC ACGCGCGGGG GTGGGCGAGC GGGCCCCGGC AGTGCACACA CACGGCAGGG GCGGGGCGAC AGATGCAGTC GTGCGCCGGA GCCCAAGCGC ACAAACGGAA AGAGCGGCCG GTGGGGGAGG GGCGGGCCCC AGCGGGGTTG GCATGCGCGC CCCCGCCCGA GGCTATAAAA CCATCGCCAC CTGCTGCCAC TAGCCAAGGG GCGCGT. __________________________________________________________________________
Dependencies are inferred from the claim text.