Specific gene probes and processes for the diagnostic investigation of Candida albicans
AssigneeBayer Akteingesellschaft
InventorsWolfgang Springer, Manfred Plempel, Antonius Lobberding
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Abstract
Polynucleotide and oligonucleotide probes are described for the diagnostic investigation of mycoses which are caused by Candida albicans. These gene probes are complementary to parts of the genome of C. albicans and are suitable for identifying C. albicans nucleic acids in any sample material, even that which has been amplified. They do not hybridise with nucleic acids from other fungi, bacteria, animals or plants. Processes are described for the diagnosis of C. albicans in clinical samples as well.
Claims22
DNA consisting of a nucleotide sequence selected from the group consisting of:
- (i) the nucleotide sequence of SEQ ID NO:
- 1;
- (ii) a nucleotide sequence fully complementary to (i);
- (iii) the nucleotide sequence of a restriction enzyme digestion fragment of (i) or (ii), said restriction enzyme digestion fragment being specific for Candida albicans;
- and
- (iii) the nucleotide sequence of a 100 mer continuous nucleotide fragment of (i) or (ii), said 100 mer continuous nucleotide fragment being specific for Candida albicans.
DNA consisting of:
- (i) the 2.6 kb ClaI digestion fragment of plasmid 436-1;
- (ii) DNA fully complementary to (i);
- and
- (iii) DNA consisting of a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 25-38.
DNA consisting of a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 39-44.
A method of detecting the presence of Candida aIbicans in a clinical sample comprising:
- (i) isolating total DNA from said sample;
- (ii) combining said total DNA with a first gene probe containing a label and a nucleotide sequence specific for Candida albicans under stringent conditions so that said gene probe hybridizes specifically to DNA which may be present from Candida albicans;
- (iii) combining said total DNA with a second gene probe containing a label and a nucleotide sequence specific for Candida albicans under stringent conditions so that said gene probe hybridizes specifically to DNA which may be present from Candida albicans;
- and
- (iv) detecting either label to give an indication of the presence of Candida albicans in said clinical sample;
- wherein steps (ii) and (iii) are carried out in any order or simultaneously;
- wherein said nucleotide sequence of said first gene probe which is specific for Candida albicans consists of a Candida albicans-specific 10 mer to 100 mer continuous fragment of SEQ ID NO:
- 1 or a nucleotide sequence fully complementary thereto;
- and
- wherein said nucleotide sequence of said second gene probe which is specific for Candida aIbicans consists of a Candida aIbicans-specific 10 mer to 100 mer continuous fragment of the 2.6 kb ClaI digestion fragment of plasmid 436-1 or DNA fully complementary thereto.
Dependencies are inferred from the claim text.