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US 5,489,510 AGranted
Method for visual indication of cholesterol on skin surface agents used therefor and methods for producing such agents
Assignee2860601 Canada Inc.
InventorsJury M. Lopukhin, Viktor V. Zuevsky, Alexander B. Rabovsky, Irina P. Andrianov, deceased
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Abstract
The invention is a non-invasive diagnostic test which is performed on the surface of the skin. This test indicates skin cholesterol levels which can provide information about the extent of aortic atherosclerosis. The invention also relates to reagents in the form of affino-enzymatic test compounds for use in the diagnostic test.
Claims38
A method of detecting the presence of free cholesterol in a warm blooded animal comprising:
- (a) contacting the skin of the warm blooded animal, in at least one location, with a composition comprising an affino-enzymatic agent for a time sufficient to at least initiate binding of the affino-enzymatic agent with cholesterol of the skin;
- (b) contacting at least one location on the skin which had been contacted with the affino-enzymatic agent, with an indicating agent D which reacts with the affino-enzymatic agent to provide a visual color change corresponding to the presence of affino-enzymatic agent bound to cholesterol of the skin.
A method for screening for cholesterol on a patient's skin to determine whether such patient belongs to one of the following groups:
- (a) atherosclerotic group;
- (b) risk group;
- or
- (c) normal group,
- which method comprises applying to the palm of said patient three different concentrations of a composition comprising an affino-enzymatic agent for a time sufficient to at least initiate binding of the affino-enzymatic agent with cholesterol of the skin;
- after one minute incubation washing to remove excess agent not bound to skin cholesterol, applying to the same skin area an indicating agent which reacts with the affino-enzymatic agent to provide a visual color change corresponding to the presence of affino-enzymatic agent bound to cholesterol of the skin and determining from any stain which forms to which group said patient belongs, by comparison with the stains produced on patients using the same concentrations and known to be classified in the aforesaid groups.
3 more elements
Dependencies are inferred from the claim text.