Density gradient medium for the separation of cells
AssigneeApplied Imaging Corporation
InventorAlexander M. Saunders
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Abstract
A density gradient medium for the isolation and enrichment of rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The medium comprises a colloidal density gradient medium dispersed in a meltable gel. In one aspect of the invention, the density gradient medium is hypertonic to facilitate separation of maternal red blood cells from fetal blood cells.
Claims13
A medium for centrifugation of a cell population, the medium comprising a colloid dispersed in a meltable water-soluble gel, wherein the colloid is capable of maintaining the cell population in a substantially unaggregated state, and wherein the medium comprises a plurality of layers, each layer having a different density.
A medium for centrifugation of a cell population, the medium comprising a colloid dispersed in a meltable water-soluble gel, wherein the colloid is capable of maintaining the cell population in a substantially unaggregated state, and wherein the medium comprises a plurality of layers, each layer having a different density, and wherein the meltable gel is selected from the group consisting of agar, agarose, low melting point agarose, alginic acid, carrageenin, pectin and gelatin.
A density gradient medium for the density separation of cells in a cell population, the density gradient medium comprising a plurality of layers of a colloid dispersed in a meltable gel, wherein the colloid is capable of maintaining the cell population in a substantially unaggregated state, the layers being enclosed within a vessel having a top and a bottom, the top having an aperture, and wherein the density of the layers increases from the top of the vessel to the bottom of the vessel.
A density gradient medium for the density separation of cells in a cell population, the density gradient medium comprising a plurality of layers of a colloid dispersed in a meltable gel, wherein the colloid is capable of maintaining the cell population in a substantially unaggregated state, the layers being enclosed within a vessel having a top and a bottom, the top having an aperture, wherein the density of the layers increases from the top of the vessel to the bottom of the vessel and wherein the meltable gel is selected from the group consisting of agar, agarose, low melting point agarose, alginic acid, carrageenin, pectin and gelatin.
Dependencies are inferred from the claim text.