Bandage for continuous application of biologicals
AssigneesThe MetroHealth System, The University of Akron, Case Western Reserve University
InventorsRichard L. Eckert, Daniel J. Smith, Irwin Schafer
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Abstract
The present invention provides a biological bandage, comprising an envelope enclosing cells which secrete biologically active cellular products such as growth factors, which promote the healing of wounds. The envelope is further comprised of a permeable bottom membrane through which the cellular product diffuses, and a top membrane. Preferably the bandage has a separator interposed between the two membranes. This invention also relates to a method for treating wounds. The bandage provides a continuous, uniform source of fresh cellular product.
Claims31
A kit for forming a bandage, containing cells that secrete a wound healing factor comprising:
- a. a container containing cells that secrete a wound healing factor;
- and
- b. a bandage comprising:
- i. a first membrane, being gas permeable and fluid impermeable, and
- ii. a second membrane, being permeable to the wound healing factor, said first and second membranes each having a perimeter, and being joined at the perimeter so as to define a chamber for receiving said cells.
A bandage containing cells that secrete wound healing factors, comprising:
- a. an envelope, comprising a gas permeable and fluid impermeable top membrane and a bottom membrane permeable to the wound healing factor, and a chamber therebetween, wherein the top membrane is interconnected to the bottom membrane to provide a leakproof seal;
- b. cells that secrete a wound healing factor, said cells located within said chamber;
- c. media, for substaining said cells, disposed within said chamber, and surrounding said cells;
- and
- d. a wound dressing disposed on the bottom side of said bottom membrane.
A bandage for containing cells that secrete wound healing factors comprising
- a. an envelope, comprising:
- i. a gas permeable and fluid impermemable top membrane and
- ii. a bottom membrane permeable to the wound healing factor, and a chamber therebetween, wherein each of said membranes has a perimeter and wherein the perimeter of said top membrane is interconnected to the perimeter of said bottom membrane to provide a leakproof seal;
- iii. a separator, interposed between said perimeter of said first membrane and said perimeter of said second membrane;
- b. cells that secrete a wound healing factor, said cells located within said chamber, and,
- c. media, for sustaining said cells, disposed within said chamber, and surrounding said cells.
The bandage as defined in claim, 16 or 19 for producing human epidermal growth factor, wherein said cells contain a plasmid comprising:
- an epidermal growth factor gene sequence comprising the following elements in the 5' to 3' direction said elements being operably linked:
- (1) promotor;
- (2) Ig leader sequence;
- (3) human epidermal growth factor gene;
- and,
- (4) terminator.
Dependencies are inferred from the claim text.