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AssigneesThe Board of Trustees of the Leland Stanford Junior University, Children's Medical Center Corporation
InventorsScott Saunders, Merton Bernfield, Masato Kato
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A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly, where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.
A proteoglycan recombinantly-produced by a transformed eukaryotic host cell capable of producing heparan sulfate glycosaminoglyycans, which proteoglycan has a heparan sulfate glycosaminoglyycan chain attached thereto, wherein the proteoglycan is selected from
A soluble proteoglycan recombinantly produced by a transformed eukaryotic cell capable of producing heparan sulfate glycosaminoglycans, which proteoglycan comprises at least one heparan sulfate attachment sequence represented by SEQ ID NO:
A purified, soluble portion of a mammalian syndecan, comprising a core polypeptide having an amino acid sequence corresponding to a fragment of SEQ. ID NO:. 2 ranging between a fragment represented by Gln23Gly50 to a fragment represented by Gln23-Gln210, and a heparan sulfate glycosaminoglycan chain covalently linked thereto which syndecan is isolated free of any biological macromolecules which are present in a natural source of the proteolglycan.
A fusion protein comprising a functional heparan sulfate attachment sequence of SEQ ID NO:
A syndecan fusion protein comprising, a first polypeptide portion having an amino acid sequence from a syndecan protein and including a heparan sulfate attachment sequence represented by SEQ ID NO:
Dependencies are inferred from the claim text.