DNA clone encoding an ethylene-forming enzyme, constructs, plant cells and plants based thereon
AssigneeZeneca Limited
InventorsColin R. Bird, John A. Ray, Wolfgang W. Schuch
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Abstract
DNA clones, e.g. M13-1, comprising at least part of a gene derived from melon that encodes ethylene-forming enzyme. Such clones may comprise DNA constructs including plant promoters capable of expressing RNA in plant cells. Such constructs may be used to inhibit production of ethylene-forming enzyme in transformed plants, and thereby to produce slower-ripening fruit, particularly melons. The clones may be obtained by PCR using specific oligo-nucleotide primers.
Claims5
A DNA clone comprising a DNA fragment that encodes ethylene-forming enzyme, said DNA fragment having the sequence of SEQ ID NO:1 found in clone M13-1.
A DNA clone comprising a DNA fragment having the sequence of SEQ ID NO:1 found in clone M13-1, in which the DNA fragment is preceded by a transcriptional initiation sequence functional in plants and is operatively joined in an antisense orientation with respect to the transcriptional initiation sequence so that the clone can generate in plant cells RNA antisense to mRNA for ethylene-forming enzyme.
Dependencies are inferred from the claim text.