Synthetic media compositions and methods for inactivating bacteria and viruses in blood preparations with 8-methoxypsoralen
AssigneeSteritech, Inc.
InventorsLily Lin, Lawrence Corash, Stephen Isaacs, George Cimino
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Abstract
Methods and compositions are described for treating contaminants in material intended for in vivo use, and in particular blood and blood products for human use. Compositions of synthetic media are described which optimize conditions for inactivation of contaminants in blood cell preparations, using 8-methoxypsoralen, prior to long term storage and transfusion.
Claims20
A method of inactivating bacteria in blood preparations, comprising:
- a) providing, in any order, i) 8-methoxypsoralen;
- ii) photoactivating means for photoactivating 8-methoxypsoralen;
- and iii) a platelet preparation suspected of being contaminated with bacteria in a synthetic media;
- b) adding said 8-methoxypsoralen to said platelet preparation at a final concentration of between approximately 3 and 30 ug/ml;
- and
- c) photoactivating said 8-methoxypsoralen, so that said 8-methoxypsoralen binds covalently to the nucleic acid of a portion of said bacteria, without causing significant damage to said platelet preparation.
A method of treating blood preparations intended for in vivo use, comprising:
- a) providing, in any order, i) an aqueous synthetic media containing 8-methoxypsoralen;
- ii) photoactivating means for photoactivating said 8-methoxypsoralen;
- iii) a blood preparation, comprising platelets suspected of being contaminated with bacteria;
- b) adding said synthetic media containing said 8-methoxypsoralen to said blood preparation;
- and
- c) photoactivating said 8-methoxypsoralen with a spectrum of electromagnetic radiation, so that said 8-methoxypsoralen binds covalently to the nucleic acid of a portion of said bacteria, without causing significant activation of said platelets.
A composition having anti-microbial properties, comprising a blood preparation in a synthetic media comprising 8-methoxypsoralen at a concentration between approximately 3 .mu.g/ml and 30 ug/ml and mannitol at a concentration of approximately 10 mM.
A method of inactivating bacteria in blood preparations, comprising:
- a) providing, in any order, i) an aqueous synthetic media comprising 8-methoxypsoralen;
- ii) photoactivating means for photoactivating said 8-methoxypsoralen;
- and iii) a blood preparation, comprising platelets suspected of being contaminated with bacteria;
- b) adding said synthetic media to said blood preparation at a final concentration of 8-methoxypsoralen of between approximately 3 and 30 ug/ml;
- and
- c) photoactivating said 8-methoxypsoralen, without limiting the concentration of molecular oxygen, so that said 8-methoxypsoralen binds covalently to the nucleic acid of a portion of said bacteria.
Dependencies are inferred from the claim text.