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US 5,480,772 AGranted
In vitro activation of a nucleus
AssigneeBrandeis University
InventorLawrence J. Wangh
Life of this patent
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Abstract
Products and methods particularly useful for activating and analyzing the nuclei and nucleic acids of human fetal red blood cells or cells found in amniotic fluid thereby facilitating prenatal screening are described. The featured products include activating egg extracts, cytostatic factor (CSF) extracts, kits containing these extracts, and a microchamber microscope slide useful in analyzing nucleus activation.
Claims5
A method for in vitro activation of a nucleus from a human fetal red blood cell or a fetal cell found in amniotic fluid comprising the steps of:
- a) separating said nucleus from said human fetal red blood cell or said fetal cell from its surrounding cytoskeleton using a protease and a non-ionic detergent to form a pretreated nucleus,
- b) contacting said pretreated nucleus with cytostatic factor extract, and
- c) contacting said pretreated nucleus from said step b with an activating egg extract to activate said pretreated nucleus.
Dependencies are inferred from the claim text.