Surface-bound fluorescent polymers and related methods of synthesis and use

A surface-bound fluorescent polymer prepared by the process comprising:

• (a.) treating a glass surface having free NH.sub.2 groups with succinic anhydride, to produce acid glass in which the free NH.sub.2 groups have been replaced with surface --NH(CO)CH.sub.2 CH.sub.2 COOH groups;
• (b.) preparing acid chloride glass by converting the surface --NH(CO)CH.sub.2 CH.sub.2 COOH groups to surface --NH(CO)CH.sub.2 CH.sub.2 COCl moieties;
• (c.) treating said acid chloride glass with N-hydroxysuccinimide, to produce NHS glass having surface ##STR20## groups;
• (d.) treating the NHS glass with triethylenetetramine, to provide aminoalkyl glass having surface ##STR21## groups;
• (e.) activating the aminoalkyl glass with acryloyl chloride to provide an activated surface having surface-bound acrylamide groups;
• and
• (f.) copolymerizing a fluorescent species having the structure ##STR22## with acrylamide in the presence of a catalyst and in the presence of the activated surface.

A process for preparing a surface-bound fluorescent polymer, comprising:

• (a.) treating a glass surface having free NH.sub.2 groups with succinic anhydride, to produce acid glass in which the free NH.sub.2 groups have been replaced with surface --NH(CO)CH.sub.2 CH.sub.2 COOH groups;
• (b.) preparing acid chloride glass by converting the surface --NH(CO)CH.sub.2 CH.sub.2 COOH groups to surface --NH(CO)CH.sub.2 CH.sub.2 COCl moieties;
• (c.) treating said acid chloride glass with N-hydroxysuccinimide, to produce NHS glass having surface ##STR23## groups;
• (d.) treating the NHS glass with triethylenetetramine, to provide aminoalkyl glass having surface ##STR24## groups;
• (e.) activating the aminoalkyl glass with acryloyl chloride to provide an activated surface having surface-bound acrylamide groups;
• and
• (f.) copolymerizing a fluorescent species having the structure ##STR25## with acrylamide in the presence of a catalyst and in the presence of the activated surface.