In a magnetic tape cassette having a metal tape guide for guiding a magnetic tape, the magnetic tape comprising a substrate and a magnetic layer provided on a front surface of the substrate, the guide being in direct contact with a back surface of the substrate, and in which the substrate is made of a material comprising one of polyethylene terephthalate and polyethylene naphthalate, the improvement wherein a surface specific resistance R.sub.s of the back surface of the substrate is at least approximately 10.sup.8 .OMEGA./cm.sup.2, the tape guide is made of a material comprising one of stainless steel and aluminum, and a surface roughness of the tape guide is such that a height of a highest one of a plurality of protrusions on the surface of the tape guide measured from a reference surface for determining a root mean square roughness value R.sub.rms is no greater than approximately 60 nm.