Displacement chromatography of proteins using low molecular weight displacers
OwnerSteven M. Cramer, James A. Moore +3inventors
InventorsSteven M. Cramer, James A. Moore, Amitava Kundu, Yufei Li, Guhan Jayaraman
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Abstract
A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight, charged species are exemplified, including aminoacids, peptides, antibiotics and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R.sup.1 is lower alkyl, n is 2 to 6, and X is a common counter anion and similar dendritic polymers based thereon.
Claims14
A method for purifying a protein comprising loading said protein in a suitable loading solvent onto an ion exchange stationary phase and displacing said protein from said stationary phase by displacement chromatography using a cationic displacer of molecular weight less than 1620.
A method for purifying a protein comprising loading said protein in a suitable loading solvent onto an ion exchange stationary phase and displacing said protein from said stationary phase by displacement chromatography using a dendritic polyelectrolyte displacer.
A compound of formula ##STR9## wherein R.sup.1 is lower alkyl, n is 2 to 6, and X is halogen, sulfate, sulfonate, perchlorate, acetate, phosphate or nitrate.
A compound of formula ##STR10## wherein R is --(CH.sub.2).sub.n --N(R.sup.1).sub.3.sup.+ X.sup.-, R.sup.1 is lower alkyl, n is 2 to 6, and X is halogen, sulfate, sulfonate, perchlorate, acetate, phosphate or nitrate.
Dependencies are inferred from the claim text.