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US 5,478,805 AGranted
Fractionation of polyalkylene oxide-conjugated hemoglobin solutions
AssigneeEnzon, Inc.
InventorsRobert G. L. Shorr, Myung-Ok P. Cho, Kwang Nho
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Abstract
Hemoglobin-containing solutions containing polyalkyene oxide-conjugated hemoglobin having a molecular weight greater than about 85,000 daltons and a degree of substitution of at least five polyalkylene oxide conjugates per hemoglobin molecule are described that are not associated with hemoglobinuria in mammals. A method of simultaneously fractionating and purifying polyalkylene oxide-conjugated hemoglobins is also disclosed.
Claims29
A method of simultaneously fractionating and purifying polyalkylene oxide-hemoglobin (PAO-Hb) conjugates, comprising:
- (a) loading a solution containing PAO-Hb conjugates onto an anion exchange medium capable of selectively binding
- (i) PAO-Hb conjugates having a molecular weight of less than approximately 85,000 daltons and a degree of substitution of less than five polyalkylene oxide conjugates per hemoglobin molecule and
- (ii) physiologically unacceptable materials selected from the group consisting of DNA, endotoxins and phspholipids;
- and
- (b) recovering the PAO-Hb conjugates not bound by said resin.
2 more elements
Dependencies are inferred from the claim text.