cDNA encoding attenuated cell culture adapted hepatitis A virus genome
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsJeffrey I. Cohen, Robert H. Purcell, Stphen M. Feinstone, John R. Ticehurst
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Abstract
A full-length cDNA copy of an attenuated, cell culture-adapted hepatitis-A virus genome has been constructed. The HAV cDNA when inserted, without the oligo (dG) oligo (dC) tails, into an RNA transcription vector yielded a plasmid designated pHAV/7. Transfection of monkey kidney cells with pHAV/7 DNA yielded HAV. Transfection with RNA transcripts produced in vitro from pHAV/7 yielded about 10-fold more HAV than transfection with pHAV/7 DNA. HAV thus produced are useful as a vaccine.
Claims4
A full-length cDNA encoding an attenuated, cell culture-adapted, infectious Hepatitis A virus, wherein said cDNA has a nucleic acid sequence corresponding to HAV HM-175/7 MK-5 except for nucleotides at positions 7027 and 7425, wherein the nucleotides of the cDNA correspond to those of wild-type HAV HM-175.
Dependencies are inferred from the claim text.