Microbially mediated degradation of nitrogen-containing phenol compounds by single bacterial isolates
AssigneeE. I. Du Pont de Nemours and Company
InventorsRichard E. Perkins, Janardhanan S. Rajan, Fateme S. Sariaslani
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Abstract
The invention relates to Arthrobacter bacterial isolates capable of degrading picric acid and related compounds to the point where no aromatic ring-containing degradation products can be detected, as determined by HPLC profiles, UV-V spectrometry, analysis of total organic carbon and mineralization of .sup.14 C-picric acid. The isolates were derived from enrichments of bacterial cultures from waste sludge by successive subculturing into medium containing picric acid as the only carbon source.
Claims9
A method of degrading an isolated sample containing tri-nitro phenol compounds comprising:
- (a) isolating a sample from a contaminated environment comprising tri-nitro phenol compounds;
- (b) inoculating a bacterial isolate into said sample, said bacterial isolate utilizing said tri-nitro phenol compounds as a carbon source resulting in the production of break-down intermediates;
- and
- (c) growing said bacterial isolate in said sample for a sufficient time period and under conditions sufficient for said growth until a decrease in the concentration of said tri-nitro phenol compounds and their break-down intermediates is achieved;
- wherein the bacterial isolate is selected from the group of Arthrobacter species consisting of ATCC 55546, ATCC 55547, ATCC 55548 and ATCC 55549.
A method of degrading an isolated sample containing tri-nitro phenol compounds comprising:
- (a) isolating a sample from a contaminated environment comprising tri-nitro phenol compounds;
- (b) inoculating a bacterial isolate into said sample, said bacterial isolate utilizing said tri-nitro phenol compounds as a carbon source resulting in the production of break-down intermediates;
- and
- c) growing said bacterial isolate in said sample for a sufficient time period and under conditions sufficient for said growth until a decrease in the concentration of said trinitro phenol compounds and their break-down intermediates is achieved, wherein the bacterial isolate is selected from the group of Arthrobacter species consisting of ATCC 55546, ATCC 55547;
- ATCC 55548 and ATCC 55549;
- and
- wherein said decrease in concentration is at least 50%.
A biologically pure Arthrobacter bacterial isolate identified as ATCC 55546.
A biologically pure Arthrobacter bacterial isolate identified as ATCC 55547.
A biologically pure Arthrobacter bacterial isolate identified as ATCC 55548.
A biologically pure Arthrobacter bacterial isolate identified as ATCC 55549.
Dependencies are inferred from the claim text.