Alkaline proteases from Bacillus pumilus

A biologically pure culture of a microorganism producing a highly alkaline protease, wherein said microorganism is a microorganism transformed to contain inexpressible form a highly alkaline protease gene isolated from Bacillus pumilus DSM 5777, said protease having either set of the following properties:

• (1) Action:
• degradation of proteins and peptides;
• (2) pH optimum:
• approximately between pH values of 10.5 to 11.0;
• (3) pH stability:
• upon incubation at 4.degree. C. for 24 hours at pH values between 9.7 to 10.8, the enzyme proves to be completely stable;
• (4) temperature optimum:
• approximately 60 .degree. C.;
• (5) temperature stability:
• upon incubation of the protease at temperatures of up to 45.degree. C. for 15 minutes, the activity of the protease is substantially 100%;
• after 15 minutes' incubation at 50.degree. C., the residual activity of the protease is at least 90% or
• (1) Action:
• degradation of proteins and peptides,
• (2) pH optimum:
• approximately between pH values of 8.5 to 9.0;
• (3) pH stability:
• upon incubation at 4.degree. C. for 24 hours between pH values of 5.5 to 10.5, the enzyme proves to be completely stable;
• (4) temperature optimum:
• approximately 50.degree. C.;
• (5) temperature stability:
• upon incubation of the protease at temperatures of up to 40.degree. C. for 15 minutes, the activity of the protease is substantially 100%;
• after 15 minutes' incubation at 45.degree. C., the residual activity of the protease is at least 95%.