Anther-specific CDNA sequences, genomic DNA sequences and recumbinant DNA sequences
AssigneeCiba-Geigy Corporation
InventorsAnnmarie B. Tuttle, Lyle D. Crossland
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Abstract
cDNA sequences are disclosed which are expressed specifically in the anther of a plant. Genomic DNA sequences corresponding to the cDNA clones are obtained using the cDNA clones as hybridization probes. Recombinant, or chimeric, DNA sequences are constructed in which the promoter sequence from anther-specific genomic clones are operatively linked to a DNA sequence coding for a desired polypeptide. Transgenic plants are made in which the chimeric DNA sequences are expressed in the anther of the transgenic plant. In a preferred embodiment, the coding DNA sequence expresses a polypeptide which will disrupt formation of viable pollen, resulting in a male-sterile plant.
Claims15
An isolated nucleotide sequence consisting of an anther-specific genomic DNA sequence selected from the group consisting of the sequences of SEQ. ID No. 16 and SEQ. ID No. 18.
A plasmid selected from the group consisting of pCIB3132 and pCIB3178.
Dependencies are inferred from the claim text.