Recombinant DNA coding for a novel protein having .beta.-1,3-glucanase activity, bacteria containing this DNA, transformed plant cells and plants
AssigneesElf Sanofi, Societe Nationale Elf Aquitaine
InventorsCatherine Sass, Jean-Jacques Leguay, Rene Grison, Alain Toppan
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Abstract
The invention provides a protein, isolated from soybean, having .beta.-1,3-glucanase activity and comprising the amino acid sequence of SEQ ID No: 1. DNA constructs encoding said protein, and transformed plants expressing the protein, are also provided.
Claims25
A DNA construct, which codes for a protein having .beta.-1,3-glucanase activity, wherein said protein comprises the following sequence (a.sub.1) (SEQ ID NO. 1) ##STR1##
An isolated, purified protein having .beta.-1,3-glucanase activity, which comprises a region having the amino acid sequence (a.sub.1) (SEQ ID NO. 1)
- ______________________________________ Ile Gly Val Cys Tyr Gly Met Leu Gly Asn Asn Leu Pro Ser Ala Asn Asp Val Ile Gly Leu Tyr Arg Ser Asn Asn Ile Lys Arg Met Arg Leu Tyr Asp Pro Asn Gln Ala Ala Leu Glu Ala Leu Arg Asn Ser Gly Ile Glu Leu Ile Leu Gly Val Pro Asn Ser Asp Leu Gln Gly Leu Ala Thr Asn Pro Asp Thr Ser Arg Gln Trp Val Gln Lys Asn Val Leu Asn Phe Trp Pro Ser Val Lys Ile Lys Tyr Val Ala Val Gly Asn Glu Val Ser Pro Val Gly Gly Ser Ser Ser Val Ala Gln Tyr Val Leu Pro Ala Ile Gln Asn Val Tyr Gln Ala Ile Arg Ala Gln Gly Leu His Asp Gln Ile Val Ser Thr Ser Ile Asp Met Thr Leu Ile Gly Asn Ser Phe Pro Pro Ser Gln Gly Ser Phe Arg Gly Asp Val Arg Ser Tyr Leu Asp Pro Ile Ile Gly Tyr Leu Val Tyr Ala Asn Ala Pro Leu Leu Val Asn Val Tyr Pro Tyr Phe Ser Tyr Thr Gly Asn Pro Arg Asp Ile Ser Leu Pro Tyr Ala Leu Phe Thr Ala Pro Asn Val Val Val Trp Asp Gly Gln Tyr Gly Tyr Gln Asn Leu Phe Asp Ala Met Leu Asp Ser Val His Ala Ala Ile Asp Asn Thr Lys Ile Gly Tyr Val Glu Val Val Val Ser Glu Ser Gly Trp Pro Ser Asp Gly Gly Phe Ala Ala Thr Tyr Asp Asn Ala Arg Val Tyr Asp Asn Leu Val Arg Arg Ala Asn Arg Gly Ser Pro Arg Arg Pro Ser Lys Pro Thr Glu Thr Tyr Ile Phe Ala Met Phe Asp Glu Asn Gln Lys Asn Pro Glu Ile Glu Lys His Phe Gly Leu Phe Asn Pro Asn Lys Gln Lys Lys. ______________________________________
An isolated, purified protein having .beta.-1,3-glucanase activity, comprising a region having the amino acid sequence (a.sub.5) (SEQ ID NO. 3)
- ______________________________________ Gln Ile Gly Val Cys Tyr Gly Met Leu Gly Asn Asn Leu Pro Ser Ala Asn Asp Val Ile Gly Leu Tyr Arg Ser Asn Asn Ile Lys Arg Met Arg Leu Tyr Asp Pro Asn Gln Ala Ala Leu Glu Ala Leu Arg Asn Ser Gly Ile Glu Leu Ile Leu Gly Val Pro Asn Ser Asp Leu Gln Gly Leu Ala Thr Asn Pro Asp Thr Ser Arg Gln Trp Val Gln Lys Asn Val Leu Asn Phe Trp Pro Ser Val Lys Ile Lys Tyr Val Ala Val Gly Asn Glu Val Ser Pro Val Gly Gly Ser Ser Ser Val Ala Gln Tyr Val Leu Pro Ala Ile Gln Asn Val Tyr Gln Ala Ile Arg Ala Gly Gly Leu His Asp Gln Ile Lys Val Ser Thr Ser Ile Asp Met Thr Leu Ile Gly Asn Ser Phe Pro Pro Ser Gln Gly Ser Phe Arg Gly Asp Val Arg Ser Tyr Leu Asp Pro Ile Ile Gly Tyr Leu Val Tyr Ala Asn Ala Pro Leu Leu Val Asn Val Tyr Pro Tyr Phe Ser Tyr Thr Gly Asn Pro Arg Asp Ile Ser Leu Pro Tyr Ala Leu Phe Thr Ala Pro Asn Val Val Val Trp Asp Gly Gln Tyr Gly Tyr Gln Asn Leu Phe Asp Ala Met Leu Asp Ser Val His Ala Ala Ile Asp Asn Thr Lys Ile Gly Tyr Val Glu Val Val Val Ser Glu Ser Gly Trp Pro Ser Asp Gly Gly Phe Ala Ala Thr Tyr Asp Asn Ala Arg Val Tyr Leu Asp Asn Leu Val Arg Arg Ala Asn Arg Gly Ser Pro Arg Arg Pro Ser Lys Pro Thr Glu Thr Tyr Ile Phe Ala Met Phe Asp Glu Asn Gln Lys Asn Pro Glu Ile Glu Lys His Phe Gly Leu Phe Asn Pro Asn Lys Gln Lys Lys Tyr Pro Phe Gly Phe Gly Gly Lys Arg Leu Gly Lys Val Val Ile Asp Asp Phe Asn Ala Thr Thr Ser Ile Lys Ser Asp Val. ______________________________________
Dependencies are inferred from the claim text.