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US 5,477,000 AGranted
Hyperproduction of shoots during a vitro regeneration of plant
AssigneeUniversity of Geulph
InventorsPraveen K. Saxena, Kamal Malik
Life of this patent
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Abstract
A plurality of viable plant regenerants can be produced by culturing an intact plant seed of in the presence of cytokinin and/or auxin growth factors. The growth factor is preferably benzylaminopurine and thiadiziron. The plant seed is preferably a pea, bean, geranium, peanut, grass pea, chickpea or lentil seed.
Claims8
A process for producing a plurality of viable plant regenerants, said plurality in excess of 14, from a single intact plant seed, said plant seed being selected from the group consisting of seeds for pea, bean, geranium, peanut, grass pea, chickpea and lentil, said process comprising:
- (i) culturing an intact plant seed under aseptic conditions in a suitable culture medium comprising a growth regulator selected from the group consisting of benzylaminopurine and thiadiziron wherein said growth regulator is present in a concentration in the amount of about 10-100 .mu.M;
- and
- (ii) continuing said culturing of the seed until said in excess of 14 regenerants are distinct and well formed.
Dependencies are inferred from the claim text.