Extended human hematopoiesis in a heterologous host
AssigneeSystemix, Inc.
InventorsHideto Kaneshima, Reiko Namikawa, Joseph M. McCune
Life of this patent
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Abstract
A human hematopoietic system is provided in an immunocompromised mammalian host, where the hematopoietic system is functional for extended periods of time. Particularly, human fetal liver tissue and human fetal thymus is introduced into an appropriate site of a young immunocompromised mouse at a site supplied with a vascular system, whereby the fetal tissue results in novel formation of functional human bone marrow tissue.
Claims6
A method of producing human bone marrow equivalent comprising:
- introducing into a vascularized site of a C.B-17 scid/scid mouse host human fetal liver and human fetal thymus tissue in juxtaposition;
- and
- maintaining said C.B-17 scid/scid mouse host in a viable state, whereby cells of said human fetal liver and human fetal thymus tissue form into bone marrow equivalent.
A method for producing mature cells of a multiplicity of human hematopoietic lineages, said method comprising:
- introducing into a vascularized site of a C.B-17 scid/scid mouse host human fetal liver and human fetal thymus tissue in juxtaposition;
- and
- maintaining said C.B-17 scid/scid mouse host in a viable state, whereby cells of said human fetal liver and human fetal thymus tissue form into bone marrow equivalent, migrate into the peripheral circulation and differentiate and mature to mature cells of a multiplicity of human hematopoietic lineages.
A C.B-17 scid/scid mouse host comprising a viable human bone marrow equivalent capable of providing mature cells of a multiplicity of hematopoietic lineages, wherein said human bone marrow equivalent is comprised of human fetal thymus and human fetal liver tissue grown in juxtaposition, wherein said bone marrow equivalent is prepared by the contiguous co-implantation of said human fetal thymus and human fetal liver tissue.
Dependencies are inferred from the claim text.