Peptide production
AssigneePPL Therapeutics (Scotland) Limited
InventorsAnthony J. Clark, Richard Lathe
Life of this patent
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Abstract
A method of producing a proteinaceous compound, involves incorporating a DNA sequence coding for polypeptide into a gene of a mammal (such as a sheep) coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal. The proteinaceous compound may be a (optionally modified) protein such as a blood coagulation factor. The DNA sequence is preferably inserted into the first exon of a gene coding for a whey protein such as beta-lactoglobulin. The proteinaceous compound will generally be recovered from milk of the female mammal, but may (for example if it is an enzyme) be used in situ.
Claims4
A female transgenic sheep whose somatic and germ cells contain a transgene construct, said transgene construct comprising:
- (a) a DNA sequence encoding a polypeptide which does not naturally occur in the milk of a non-transgenic sheep;
- and
- (b) a .beta.-lactoglobulin promoter operably linked to said DNA sequence encoding said polypeptide;
- wherein said transgene construct is integrated in such a way that said DNA sequence encoding said polypeptide is expressed in the mammary gland of said sheep to produce a proteinaceous compound comprising said polypeptide in the milk of said female transgenic sheep.
Dependencies are inferred from the claim text.