Method for culturing T precursor cells under conditions of high oxygen concentration
AssigneeJapan Tobacco Inc.
InventorYoshihiro Watanabe
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Abstract
The present invention provides culture methods of T lineage precursor cells comprising a step of culturing the T lineage precursor cells under a condition that a dissolved oxygen concentration in the nutrient medium is higher than a dissolved oxygen concentration in the nutrient medium when the medium is in contact with normal atmospheric air. According to the methods of the present invention, by using a submerged (suspension) culture which is a general method for culturing cells or tissues, the derivation of matured T cell(s) differentiated from T precursor cells in terms of their phenotypes of differentiation antigens and of their functions is realized to be accomplished.
Claims17
A method for culturing mammalian T precursor cells to produce matured mammalian T cells comprising a step of culturing a mammalian tissue containing mammalian T precursor cells together with interstitial cells of a mammalian thymus in a nutrient medium having a dissolved oxygen concentration ranging from 360 .mu.M to 855 .mu.M, to obtain matured T cells.
A method for culturing mammalian T precursor cells to produce matured mammalian T cells comprising a step of culturing a mixture of mammalian T precursor cells and interstitial cells of a mammalian thymus in a nutrient medium having a dissolved oxygen concentration ranging from 360 .mu.M to 855 .mu.M, to obtain matured T cells.
A method for culturing mammalian T precursor cells to produce mammalian matured T cells comprising a step of culturing a mixture of mammalian T precursor cells and interstitial cells of a mammalian thymus in a nutrient medium contacting a gas phase having an oxygen content ranging from 40% to 95%, to obtain matured T cells.
Dependencies are inferred from the claim text.