Method of growing and attenuating a viral agent associated with mystery swine disease
AssigneeBoehringer Ingelheim Animal Health, Inc.
InventorsDanny W. Chladek, David E. Gorcyca, Louis L. Harris
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Abstract
A viral agent that will mimic "mystery swine" disease has been isolated and described, together with a method of growing the viral agent in vitro, and a method of attenuating the virus for vaccine preparation.
Claims5
A method of growing and isolating swine infertility and respiratory syndrome virus, ATCC-VR2332, which comprises inoculating the virus on a full or partial sheet of simian cells in the presence of serum in a suitable growth medium and incubating the inoculated cell sheet at about 34.degree. C. to 37.degree. C. until CPE is observed.
A method of attenuating swine infertility and respiratory syndrome virus, ATCC-VR2332, which comprises passaging the virus through a simian cell line on maintenance medium in the presence of serum at pH about 7.6 about twenty-five time at about 35.degree.-37.degree. C. without carbon dioxide, and then passaging the resulting virus through a simian cell line on maintenance medium in the presence of serum at pH about 7.6 about twelve times at about 31.degree. C.
Dependencies are inferred from the claim text.