Pretreatment of sample for endotoxin measurement
AssigneeWako Pure Chemical Industries, Ltd.
InventorsMasakazu Tsuchiya, Kazuaki Harada
Life of this patent
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Abstract
A pretreating method for a sample such as plasma for endotoxin measurement which includes diluting the sample with a surfactant-containing aqueous solution, and subjecting the diluted sample to heat treatment can prevent influences of inhibitors, etc. present in the sample and gives high recovery of endotoxins.
Claims10
A process for pretreating a sample for endotoxin measurement, which consists essentially of diluting the sample with an aqueous solution containing a surfactant, said aqueous solution being endotoxin free and having a property of not inhibiting nor enhancing the reaction between a horseshoe crab hemocyte lysate and endotoxins, and heating the diluted sample at 60.degree. to 100.degree. C., whereby false positives in a subsequent endotoxin measurement are prevented and endotoxin recovery of 50% or more is provided in the subsequent endotoxin measurement.
A process for measuring the concentration of endotoxin in a sample with a recovery of 50% or more and without false positives, which comprises subjecting the sample prior to measuring the concentration of the endotoxin to a pretreatment consisting essentially of diluting the sample with an aqueous solution containing a surfactant, said aqueous solution being endotoxin free and having a property of not inhibiting nor enhancing the reaction between a horseshoe crab hemocyte lysate and endotoxins, heating the diluted sample at 60.degree. to 100.degree. C., and to measuring the concentration of the endotoxin.
Dependencies are inferred from the claim text.