Test for quantitative thrombin time
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsThomas J. Reid, Barbara Alving
Life of this patent
Estimating the term…
Abstract
A quantitative method for determining the plasma levels of thrombin-specific inhibitors is based on the quantitative thrombin time using plasma dilutions, excess fibrinogen and thrombin. The plasma dilutions and excess fibrinogen act in concert to eliminate the effects that coagulopathies have on standard coagulation tests. The method is relatively simple and provides superior results to standard conventional tests. The method is suitable for performance in clinical hematology laboratories on a routine basis using commercially available instrumentation.
Claims6
A method of measuring quantitative thrombin time comprising the steps of:
- (1) preparing a composition comprising plasma in buffer to obtain a concentration of about 1:10 plasma to buffer;
- (2) preparing a fibrinogen mixture comprising fibrinogen concentration of about 0.1 to 18 .mu.M fibrinogen in buffer;
- (3) preparing a composition containing a concentration of about 2.5 to 20 U/mL alpha-thrombin in buffer;
- (4) preparing a mixture comprising equal amounts of the compositions obtained in steps (1) and (2);
- (5) placing in an assay well an aliquot of the mixture prepared in step (4) and an aliquot of the mixture prepared in step (3);
- and
- (6) determining the amount of time required for clot formation.
Dependencies are inferred from the claim text.