Method using CO for extending the useful shelf-life of refrigerated red blood cells

A method for storing red blood cells which consists essentially of the steps of:

• a. forming a quantity of packed red blood cells;
• b. mixing the packed red blood cells with an anticoagulant solution which comprises glucose, adenine, and salts, forming thereby a suspension of red blood cells;
• c. cooling the suspension of red blood cells to 4.degree. C.;
• and
• d. introducing carbon monoxide into the cooled suspension of red blood cells and maintaining a sufficient quantity thereof such that the hemoglobin in the red blood cells is substantially stabilized thereby.

A method for storing red blood cells which consists essentially of the steps of:

• a. mixing a sample of whole blood containing the red blood cells to be stored with a first anticoagulant solution, forming thereby a first suspension of red cells;
• b. concentrating the red blood cells from the first suspension, forming thereby packed red blood cells;
• c. mixing the packed red blood cells so produced with a second anticoagulant solution which comprises glucose, adenine, and salts, forming thereby a second suspension of red blood cells;
• d. cooling the second suspension of red blood cells to 4.degree. C.;
• e. introducing carbon monoxide into the cooled second suspension of red blood cells and maintaining a sufficient quantity thereof such that the hemoglobin in the red blood cells is substantially stabilized thereby;
• and
• f. illuminating the cooled second suspension of red blood cells containing carbon monoxide with light having wavelengths between 260 and 480 nm to drive off the carbon monoxide before use thereof.

A method for storing red blood cells which consists essentially of the steps of:

• a. mixing a sample of whole blood containing the red blood cells to be stored with a first anticoagulant solution, forming thereby a first suspension of red cells;
• b. concentrating the red blood cells from the first suspension, forming thereby packed red blood cells;
• c. mixing the packed red blood cells so produced with a second anticoagulant solution which comprises glucose, adenine, and salts, forming thereby a second suspension of red blood cells;
• d. cooling the second suspension of red blood cells to 4.degree. C.;
• e. introducing carbon monoxide into the cooled second suspension of red blood cells and maintaining a sufficient quantity thereof such that the hemoglobin in the red blood cells is substantially stabilized thereby;
• f. illuminating the cooled second suspension of red blood cells containing carbon monoxide with light having wavelengths between 260 and 480 nm to drive off the carbon monoxide before use thereof;
• and
• g. introducing oxygen into the cooled second suspension of red blood cells containing carbon monoxide during said step of illumination thereof in order to assist in driving the carbon monoxide from the red blood cells.

A method for storing red blood cells which consists essentially of the steps of:

• a. forming a quantity of packed red blood cells;
• b. mixing the packed red blood cells with an anticoagulant solution which comprises glucose, adenine, and salts, forming thereby a suspension of red blood cells;
• c. cooling the suspension of red blood cells to 4.degree. C.;
• d. introducing carbon monoxide into the cooled suspension of red blood cells and maintaining a sufficient quantity thereof such that the hemoglobin in the red blood cells is substantially stabilized thereby;
• and
• e. illuminating the cooled suspension of red blood cells containing carbon monoxide with light having wavelengths between 260 and 480 nm to drive off the carbon monoxide before use thereof.

A method for storing red blood cells which consists essentially of the steps of:

• a. forming a quantity of packed red blood cells;
• b. mixing the packed red blood cells with an anticoagulant solution which comprises glucose, adenine, and salts, forming thereby a suspension of red blood cells;
• c. cooling the suspension of red blood cells to 4.degree. C.;
• d. introducing carbon monoxide into the cooled suspension of red blood cells and maintaining a sufficient quantity thereof such that the hemoglobin in the red blood cells is substantially stabilized thereby;
• e. illuminating the cooled suspension of red blood cells containing carbon monoxide with light having wavelengths between 260 and 480 nm to drive off the carbon monoxide before use thereof;
• and
• f. introducing oxygen into the cooled second suspension of red blood cells containing carbon monoxide during said step of illumination thereof in order to assist in driving the carbon monoxide from the red blood cells.