Method for the isolation and purification of taxol and its natural analogues
AssigneeUniversity of Florida
InventorKoppaka V. Rao
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Abstract
An improved method for isolating taxol and certain clinically important analogues of taxol from a crude extract of a naturally occuring Taxus species comprising treating the extract by reverse phase liquid chromatography on an adsorbent, causing the taxol and the taxol analogues to be absorbed on the adsorbent, and recovering taxol and the natural analogues of taxol from the adsorbent by elution with an elutant. The compounds thus isolated in pure form are taxol, taxol-7- xyloside, 10-deacetyltaxol, 10-deacetyltaxol-7-xyloside, cephalomannine, cephalomannine-7-xyloside, 10-deacetylcephalomannine-7-xyloside, baccatin III, 10-deacetylbaccatin III, baccatin VI, brevitaxane A, and taxiflorine.
Claims22
A method for isolating taxol and natural analogues of taxol, said method comprising the steps of:
- (a) treating an extract comprising taxol and the natural analogues of taxol by reverse phase liquid chromatography on a single preparative column having an adsorbent, causing said taxol and said analogues of taxol to be adsorbed on the adsorbent;
- (b) eluting, with an elutant, taxol and the natural analogues of taxol from the adsorbent;
- and
- (c) recovering taxol and the natural analogues of taxol in separate fractions of eluate;
- (d) treating with ozone an eluted fraction comprising an ozone-reactive taxol analogue obtained in step (c), wherein said ozone-reactive taxol analogue undergoes ozonolysis which permits improved separation of the taxol analogue from taxol;
- new line, insert "(e) treating the second recovered taxol and the natural analogues of taxol by a second chromatography step."
Dependencies are inferred from the claim text.