Non-tumorigenic cell lines for expression of genes
AssigneeState of Oregon
InventorDavid W. Barnes
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Abstract
Non-tumorigenic cell lines capable of indefinite growth in serum-free media are disclosed. The cell lines are capable of expressing exogenously introduced genes, and may be derived from mouse embryo cells. Methods for producing proteins utilizing these cell lines, and methods for selectively controlling the growth of the cell lines are also disclosed.
Claims9
A non-tumorigenic murine cell line which exhibits a predominantly diploid karyotype and is capable of indefinite growth in serum-free media, said cell line being further capable of expressing exogenously introduced genes.
A non-tumorigenic cell line SFME Swiss (ATCC No. CRL 9391).
A non-tumorigenic cell line SFME Balb/C (ATCC No. CRL 9392).
A method for selectively controlling the growth of a non-tumorigenic murine cell line which exhibits a predominantly diploid karyotype and is capable of indefinite growth in serum-free media, comprising:
- growing said cell line in a serum-free media;
- and
- supplementing said media with a selected amount of a biological substance selected from the group consisting of serum, plasma, conditioned cell culture medium, tissue extracts and purified or partially purified components thereof.
Dependencies are inferred from the claim text.