N-acetyl-.beta.-glucosaminidase isolated from Trichoderma harzianum
AssigneeCornell Research Foundation, Inc.
InventorsGary E. Harman, Matteo Lorito, Antonio Di Pietro, Christopher K. Hayes
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Abstract
N-acetyl-.beta.-glucosaminidase isolated from Trichoderma harzianum strain P1 having accession No. ATCC 74058 has molecular mass of 72 kDa and isoelectric point of 4.6. Glucan 1,3-.beta.-glucosidase isolated from Trichoderma harzianum strain P1 has molecular mass of 78 kDa and isoelectric point of 6.2. Combinations of endochitinase, chitin 1,4-.beta.-chitobiosidase, N-acetyl-.beta.-glucosaminidase and glucan 1,3-.beta.-glucosidase have synergistic antifungal effect.
Claims1
An essentially pure enzyme having N-acetyl-.beta.-glucosaminidase activity, which is derived from Trichoderma harzianum strain P1 ATCC 74058 and which has a molecular mass of 72 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis after the enzyme was prepared under reducing conditions, from a regression based on the log of molecular weight of standard proteins, and an isoelectric point of 4.6 as determined by isoelectric focusing electrophoresis from a regression of distance versus isoelectric point of standard proteins.
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