Immobilized proteins in cotton fiber
AssigneeAgracetus, Inc.
InventorsJohn Maliyakal, Kenneth A. Barton
Life of this patent
Estimating the term…
Abstract
Transgenic cotton plants have been created which expressed an immobilized protein in the cotton fiber cells. The cotton fiber can be recovered from such transgenic cotton plants and then used as a substrate for fixing immobilized protein for use in industrial or laboratory processes. Using an enzyme expressed inside cotton fiber, it is possible to fabricate a convenient reaction column by simply packing the cotton fiber carrying the enzyme into a column and passing substrate therethrough.
Claims5
A method for creating an immobilized enzyme on a support comprising the steps of
- creating a genetic construction for the expression of the enzyme in a cotton plant, the genetic construction including a coding region for the enzyme and a promoter effective to express the coding region in the seed floss fiber cells of the cotton plant, the enzyme being operable to catalyze a reaction on substrates under the conditions prevalent in a seed floss fiber cell;
- genetically transforming the genetic construction into the inheritable genetic material of the cotton plant;
- cultivating the cotton plant;
- harvesting the seed floss fiber produced by the cotton plant with the enzyme immobilized therein, the fiber serving as a support for the enzyme;
- and
- performing a reaction with the enzyme in its active form in the harvested fiber by exposing the harvested fiber to substrates which can be catalyzed by the enzyme.
A method of obtaining enzyme immobilized in a support comprising the steps of
- cultivating a transgenic cotton plant comprising in its inheritable genetic material a foreign genetic construction causing expression of the enzyme in the seed floss fiber cells of the cotton plant, the enzyme being operable to catalyze a reaction on substrates at a pH and under conditions found in a seed floss fiber cell;
- recovering the seed floss fiber from the cotton plant;
- and
- using the recovered cotton fiber with the enzyme therein to catalyze the reaction catalyzed by the enzyme by exposing the recovered cotton fiber to the substrates for the reaction catalyzed by the enzyme.
A method of obtaining enzyme immobilized in a support comprising the steps of
- cultivating a transgenic cotton plant comprising in its inheritable genetic material a foreign genetic construction causing expression of the enzyme in the lumen of the seed floss fiber cells of the cotton plant, the enzyme being operable to catalyze a reaction on substrates at a pH and under conditions found in the lumen of a cotton seed floss fiber cell;
- recovering the seed floss fiber from the cotton plant;
- and
- using the recovered cotton fiber with the enzyme therein to catalyze the reaction catalyzed by the enzyme by exposing the recovered cotton fiber to an aqueous solution containing the substrates for the reaction catalyzed by the enzyme.
Dependencies are inferred from the claim text.