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US 5,474,923 AGranted
Method for the biological preparation of hydroxyvitamin D compounds
AssigneeMercian Corporation
InventorsKoji Takeda, Kiyoshi Kimura, Kazuhiko Okamura, Rokuro Okamoto, Joji Sasaki, Takashi Adachi, Sadafumi Omura
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Abstract
A method for introducing hydroxyl groups into vitamin D compound at the 1.alpha.- and/or 25-positions in the presence of a cyclodextrin compound by the use of a reaction mixture containing a microorganism being capable of hydroxylating vitamin D compound or a enzyme produced from the microorganism, is disclosed.
Claims13
A method for the biological preparation of a 25-hydroxyvitamin D compound which comprises:
- adding a vitamin D compound, having a hydrogen atom at the 25-position, and one or more cyclodextrin compound, to a reaction mixture containing a microorganism or an enzyme extract thereof which is capable of hydroxylating the vitamin D compound, and
- recovering the 25-hydroxyvitamin D product.
A method for the biological preparation of a 1.alpha.,25-hydroxyvitamin D compound which comprises:
- adding a vitamin D compound, having hydrogen atoms at the 1.alpha.- and 25-positions, and one or more cyclodextrin compounds to a reaction mixture containing a microorganism or an enzyme extract thereof which is capable of hydroxylating the vitamin D compound, and
- recovering the 1.alpha.,25-hydroxyvitamin D product.
Dependencies are inferred from the claim text.