Modified cyclodextrin glycosyltransferases for producing .gamma.-cyclodextrins
AssigneeConsortium fur Elektrochemische Industrie GmbH
InventorsGeorg E. Schulz, Anton Candussio
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Abstract
A cyclodextrin glycosyltransferase (CGTase) which, in the conversion of starch or starch-like substrates to CD, produces .gamma.-CD in an increased amount, and whose protein sequence, in the region between amino acid position 180 and amino acid position 240, contains the amino acid sequence (SEQ ID NO: 1), where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and Xxx denotes a natural amino acid.
Claims8
A cyclodextrin glycosyltransferase (CGTase) which, in the conversion of starch or starch-like substrates to cyclodextrin (CD), produces .gamma.-CD in an increased amount over its natural production, comprising a CGTase whose protein sequence, in the region between amino acid position 180 and amino acid position 240, contains the amino acid sequence SEQ ID NO:
- 1, where position 1 of the CGTase's protein sequence is the beginning of the signal peptide of the CGTase and Xaa of SEQ ID NO:
- 1 denotes a natural amino acid other than Tyr;
- and wherein Xaa is selected from the group consisting of Trp and Ser.
A process for preparing CGTases such that they produce .gamma.-CD in an increased amount over their natural production, comprising mutating the DNA sequence of a gene encoding a .beta.-CGTase or .gamma.-CGTase by means of mutagenesis which results in replacing the Tyr in the region between amino acid positions 180 and 240 in the sequence motif of amino acids 3 through 6 of SEQ ID NO:
- 4 of the .beta.-CGTase or .gamma.-CGTase by another natural amino acid.
Dependencies are inferred from the claim text.