Multilayer analytical element for salicylate assay
AssigneeEastman Kodak Company
InventorsThomas C. Arter, Karen L. Warren, Harold C. Warren, III
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Abstract
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support: a) a spreading layer, b) a dye layer having a pH greater than 6.5 and comprising tyrosinase and a water-insoluble hydrazone coupling agent, c) a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and d) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), wherein the spreading layer comprises valproic acid, 8-anilino-1-naphthalenesutfonic acid (ANS) or the magnesium salt of 8-anilino-1-naphthalenesulfonic acid.
Claims7
A multilayer analytical element comprising a support coated in the following order, top down to the support:
- a) a spreading layer,
- b) a dye layer having a pH greater than 6.5 and comprising tyrosinase and a water-insoluble hydrazone coupling agent,
- c) a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and
- d) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), wherein the spreading layer comprises a compound selected from the group consisting of valproic acid, 8-anilino-1-naphthalenesulfonic acid (ANS) and the magnesium salt of 8-anilino-1-naphthalenesulfonic acid.
Dependencies are inferred from the claim text.