Process for preparing purified syphilis antigen from Treponema palljdum
AssigneeSekisui Chemical Co., Ltd.
InventorsFumio Ishikawa, Kouhei Nagahara, Mie Matsumoto
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Abstract
A process for preparing a purified syphilis antigen from Treponema pallidum is presented. The process comprises the steps of obtaining an extract from Treponema pallidum, adsorbing the extract onto hydroxyapatite gel and eluting the antigen in the presence of a surfactant. The preferred surfactant is octylglucopyranoside, A diagnostic agent is prepared which comprises the purified syphilis antigen adsorbed on an inert carrier, which carrier at least partially a hydrophobic carrier to which the antigen is adsorbed.
Claims6
A process for preparing a purified syphilis antigen which comprises adsorbing an extract originated from Treponema pallidum on a hydroxyapatite gel, eluting with an aqueous medium containing octylglucopyranoside and recovering the purified syphilis antigen.
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