Selective immunoassay for IL-1 .beta.
AssigneeCistron Biotechnology, Inc.
InventorPeter J. Lisi
Life of this patent
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Abstract
The subject invention concerns a selective, sensitive, and highly reliable immunoassay for detecting human IL-1 .beta. in cultured mononuclear cells or human body fluids. It is a competitive immunoassay which is useful in diagnostic work to detect IL-1.beta. selectively, for the first time, from among similar lymphokines and other substances known to interfere with bioassays for IL-1.
Claims21
An immunoassay method for the detection of human interleukin-1.beta. (IL-1.beta.) comprising:
- (a) contacting a sample suspected of containing IL-1.beta. with labeled recombinant human IL-1.beta. (rIL-1.beta.) and a polyclonal rabbit antibody that binds specifically to IL-1.beta. to form an immune complex, wherein said antibody does not bind human IL-1.alpha., human IL-2, human tumor necrosis factor-.alpha., or human .gamma.-interferon, wherein said antibody binds to human IL-1.beta. in the presence or absence of phytohemagglutinin or endotoxin, and wherein said antibody blocks the biological activity of human IL-1.beta. but not bind to or block the biological activity of IL-1.alpha.;
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- (b) determining the presence of said immune complex to thereby detect human IL-1.beta. in said sample.
A kit for performing an immunoassay comprising:
- (a) a container of polyclonal anti-IL-1.beta. that binds specifically to human IL-1.beta. and not bind to human IL-1.alpha., human IL-2, human minor necrosis factor-.alpha., or human .gamma.-interferon, and that binds to human IL-1.beta. in the presence or absence of phytohemagglutinin or endotoxin;
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- (b) a container of rIL-1.beta. standard.
A polyclonal rabbit antibody that binds specifically to human IL-1.beta. and does not bind to human IL-1.alpha., human IL-2, human tumor necrosis factor-.alpha., or human .gamma.-interferon, and that binds to human IL-1.beta. in the presence or absence of phytohemagglutinin or endotoxin, and wherein said antibody blocks the biological activity of human IL-1.beta. but not bind to or block the biological activity of IL-1.alpha..
A method for producing a polyclonal antibody to human IL-1.beta., comprising the steps of immunizing an animal with an effective amount of purified human IL-1.beta., or a fragment thereof, and isolating serum containing said antibody from said immunized animal, wherein said antibody binds specifically to human IL-1.beta. and does not bind to human IL-1.alpha., human IL-2, human tumor necrosis factor-.alpha., or human .gamma.-interferon, binds to human IL-1.beta. in the presence or absence of phytohemagglutinin or endotoxin, and wherein said antibody binds to human IL-1.beta. and blocks the biological activity of human IL-1.beta. and does not block biological activity of IL- 1.alpha..
Dependencies are inferred from the claim text.